A METHOD FOR DETERMINING THE IMMUNOREACTIVITY OF A RADIOLABELED ANTIBODY TO AN ANTIGEN-POSITIVE CELL

摘要

(i) mixing a chelator-conjugated antibody such as hereinbefore described with a solution of the kind such as hereinbefore described containing a radiolabel; characterized in that said chelator is selected from the group consisting of MX-DTPA, phenyl-DTPA, benzyl-DTPA and DOTA; (ii) incubating the mixture for a relatively short amount of time to produce a radiolabeled antibody; (iii) mixing and incubating at least one aliquot of a radiolabeled antibody with at least one aliquot of antigen-positive cells; (iv) mixing and incubating at least one aliquot of a radiolabeled antibody identical to the aliquot of step (iii) with at least one aliquot of dilution buffer of the same volume as the aliquot of target cells in step (iii) as a control; (v) pelleting the antigen-positive cells by centrifugation; (vi) measuring the radioactivity in the supernatant of the pelleted antigen-positive cells and the control; and (vii) comparing the quantity of radioactivity in the cell supernatant to the quantity of radioactivity in the control.