A PROCESS FOR THE PREPARATION OF BRUGIA MALAYI MICROFILARIAL EXCRETORY-SECRETORY (mfES-22) GLYCOPROTEIN.

摘要

"A process for the preparation of brugia malayi microfilarial excretory-secretory (mfES-22) glycoprotein" This invention relates to a process for the preparation of brugia malayi microfilarial excretory-secretory (mf ES-22) glycoprotein for use in diagnosis and monitoring of active filarial infection comprising. Collecting brugia malayi microfilariae by lavage of the peritoneal cavities of jirds-with an intraperitoneal filarial infection of 3 months or more duration. Centrifuging peritoneal fluid at lOOOg for 15 mins, suspending the mf pellet in sterile normal saline (0.9% NaC1) and repeated washing with RPMI-1640 medium. Plating the mf at 37°C for 1 hr to remove peritoneal exudate cells. Maintaining about ten lakhs of mf in RPMI- 1640 medium supplemented with malic acid, a keto glutaric acid, D-Succinic acid and fumeric acid at concentrations of 670, 370, 60 and 55 mg per litre respectively and sucrose and fructose at concentrations of 26, 680 and 400 mg per litre respectively, 0.1 mM phenyl methyl sulphonyl fluoride at temperature of 28-37°C in an atmosphere of 5% CO2 for 48 hrs. Centrifuging at 1000g for 20 mins followed by concentrating the supernatant by ultra membrane filtration using Dialysis tubing and dialysing against 0.1M sodium phosphate buffer, pH 7.2. (MONA SAINI) OF L.S. DAVAR & CO., APPLICANTS ATTORNEY