AN ASSAY SYSTEM FOR TRANSFORMING GROWTH FACTOR-BETA AND SMAD ACTIVITY AND ITS APPLICATION

摘要

One analysis system is arranged to simplify experimentation and measures a large amount of sample for measuring Smad activation by a TGF-β. (i) Smad activation-dependence reporter expression vector includes a nucleic acid molecules, its DNA sequence that it is connected to Smad transcription factors, (ii) the luciferase gene of the 3' terminals of nucleic acid molecules is connected to, (iii) the one antibiotic-resistant gene as a selection probe. Antibiotic-resistant gene is hygromycine resistant genes. A kind of method, the quantity for measuring activation or Smad transcription factors or TGF-β include: the step for handling TGF-β inhibitor or activator into a gene transfecting cell line; One step of the luciferase activation for the cell line that measurement processing is crossed; A kind of method includes: to handle TGF-β inhibitor or activator enters a step of gene transfecting cell line for screening a kind of anticancer agent, anti-inflammatory drug or anti-fibrosis drug; Compare a step of luciferase activation between gene transfecting cell line and control unit.