METHOD FOR KARYOTYPING OF SOLID TUMOR CELLS USING IN SITU CULTURE THEREOF

摘要

A kind of method, the culture in situ solid tumor cell and karyotyping are arranged to enhance the culturability of solid tumor cell and shorten culture time. A kind of method, the culture in situ solid tumor cell include: the fragment of centrifugation solid tumor mass to collect a step of solid tumor cell; Culture unit induces the step to the mid-term or sliding that use a cover slip as a supporter; One step of the fixation of execution unit. The condition of culture is below 3%~7% CO2 concentration and at 30 degrees Celsius~40 degrees Celsius