CHARACTERIZATION OF SINGLE STRANDED NUCLEIC ACID BY MELTING ANALYSIS OF SECONDARY STRUCTURE USING DOUBLE STRANDED NUCLEIC ACID-SPECIFIC DYE

摘要

PPROBLEM TO BE SOLVED: To provide a novel method for characterizing nucleic acids in relating to identification and characterization of the nucleic acids and modification thereof. PSOLUTION: A nucleic acid is combined with a double stranded nucleic acid-specific dye to form a detectable complex between the dye and one or more double stranded structures within the nucleic acid. Subsequently, the combination is exposed to varying temperatures and the fluorescence emission of the dye is measured to determine the melting temperature. Subsequently, in some embodiments, the melting temperature profile is compared with melting temperature profiles generated for other nucleic acids to discriminate differences between the compared nucleic acids. PCOPYRIGHT: (C)2010,JPO&INPIT