3-dimensional vaginal tissue model, including the immune cells

摘要

Are cultured in a liquid interface, cervical consists of immune cells and vaginal epithelial cells - Air - vaginal tissue equivalent is disclosed. Tissue equivalent, may be infected with a sexually transmitted pathogen virus (HIV, for example), bacteria, and fungi or parasites. The tissue equivalent is, it is possible to carry out the reaction-stimulated or allergic type reaction. And a nucleated suprabasal cells and nucleated basal layer cells, further, on the outside of the base layer to reduce the content of the nucleus and increases the content of glycogen progressively, tissue equivalent has a cell layer is characterized by. The basal layer in the first and immune cells of the tissue equivalent is located in the basal layer. A method for producing a tissue equivalent is disclosed. To provide immune cells and vaginal epithelial cells that are seeded on the porous support cells, and the method, under appropriate conditions, air differentiation - comprising co-cultured in liquid interface. One such method disclosed is the production of tissue equivalents in serum-free medium. a particular cell tissue equivalent is formed, preferred specific components of the culture medium tissue equivalents are produced are provided and also. In addition, cervical made by the methods disclosed herein - vaginal tissue equivalents are also disclosed.