Vector and its utilization method for analysis of promoter activity of high plant

摘要

Topic Development of the knock in method with the homology rearrangement for analyzing qualitatively and quantitatively was strongly desired the promoter with the high plant. Solutions This invention was formed the below-mentioned element by from RB including in the below-mentioned order with RB and LB of T DNA origin, it is the vector for analysis of promoter activity of high plant.(1) revelation it existed possibly, the first negative selection marker gene(2) the first homologous rearrangement territory which consists of the base arrangement 500 - 6000bp 3 ' end of the target promoter in the landlord genome is included(3) the start codon 3 ' from end of the aforementioned promoter interval of desire the reporter gene which leaves(4) revelation the positive selection marker gene which it exists possibly(5) the second homologous rearrangement territory which consists of the base arrangement 500 - 6000bp it follows to the aforementioned first homologous rearrangement territory in the landlord genome(6) revelation it existed possibly, the second negative selection marker gene which is possible to be identical with the first negative selection marker gene Connect the useful protein gene and the gene which relates to useful matter production, it is possible and the like by high with such as the organization which it is easy to extract making use of this vector searching the promoter who is revealed, to produce the useful protein in large quantities. Selective figure It is not