IMPROVED METHOD FOR THE PREVENTION OF CARRYOVER CONTAMINATION IN NUCLEIC ACID AMPLIFICATION TECHNOLOGIES

摘要

An improved method of preventing carryover contamination of anamplification reaction involves treating uracil-containing DNA with uracil-N-DNAglycosylase and heating the DNA in the presence of polyamines, such asspermidine,spermine and the like. Alternatively, the after treatment with uracil-N-DNAglycosylase, the reaction is further incubated with an enzyme having AP lyaseactivity.