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METHOD FOR DETECTING HUMAN METAPNEUMOVIRUS AND OTHER RESPIRATORY VIRAL AGENTS IN A TEST SAMPLE

机译:检测样品中人间质肺病毒和其他呼吸道病毒的方法

摘要

A method and kit for detection and identification in a test sample of hMPV,are described, as is a set of sequencesand their use as amplification primers for hMPV. hMPV amplification accordingto the present invention is compatible withdetection of other viruses that cause respiratory tract infections, as well aswith genotyping of hMPV present in the sample. Aftersubjecting the test sample to amplification with primers specific for eachvirus, single-stranded DNA is obtained from the amplificationproducts, and hybridised to virus-specific probes.
机译:用于检测和鉴定hMPV样品的方法和试剂盒,被描述为一组序列及其用作hMPV的扩增引物。 hMPV扩增根据本发明与检测其他引起呼吸道感染的病毒,以及样本中存在hMPV基因分型。后用对每种样品特异的引物对测试样品进行扩增病毒,通过扩增获得单链DNA产品,并与病毒特异性探针杂交。

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