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IN VIVO EVOLUTION OF HYDROGENASES USING A HYDROGEN-SENSING SYSTEM
IN VIVO EVOLUTION OF HYDROGENASES USING A HYDROGEN-SENSING SYSTEM
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机译:利用氢感测系统进行氢酶的体内进化
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摘要
Provided herein are methods for measuring H2 production by a hydrogenase. Also provided are methods for evolving a hydrogenase or hydrogen-sensing system by comparing the level of production of H2 by the hydrogenase to the level Of H2 produced by a wild-type hydrogenase cultured under the same conditions and selecting a microorganism with increased H2 production over the H2 production by a microorganism having a wild-type hydrogenase. Further provided herein are microorganisms and plasmids comprising a hupSL promoter coupled with a reporter gene.
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