首页> 外国专利> STN GENE OLIGONUCLEOTIDE PRIMERS FOR DETECTING SALMONELLA SPECIES AND DETECTION PROCESS USING THE SAME

STN GENE OLIGONUCLEOTIDE PRIMERS FOR DETECTING SALMONELLA SPECIES AND DETECTION PROCESS USING THE SAME

机译:STN基因寡核苷酸检测沙门氏菌的物种并使用相同的检测方法

摘要

The present invention relates to oligonucleotide primers having SEQ ID NOs. 1 to 21 specific for Salmonella enterotoxin gene (stn) gene, useful for rapid and specific screening of Salmonella. The present invention relates to a process for the rapid and specific detection of Salmonella enterotoxin gene (stn) gene in a subject for the presence of parasite Salmonella using oligonucleotide primers having SEQ ID NOs. 1 to 21 for Polymerase Chain Reaction (PCR), said process comprising the steps of preparing a DNA template, amplifying the template using the primers by PCR, running the PCR products on gel, and detecting the Salmonella.
机译:本发明涉及具有SEQ ID NOs的寡核苷酸引物。沙门氏菌肠毒素基因(stn)基因特异的1到21,可用于沙门氏菌的快速和特异性筛选。本发明涉及一种方法,该方法使用具有SEQ ID NOs的寡核苷酸引物在受试者中快速和特异性地检测沙门氏菌肠毒素基因(stn)基因是否存在寄生虫沙门氏菌。聚合酶链反应(PCR)的图1至21,所述方法包括以下步骤:制备DNA模板,通过PCR使用引物扩增模板,在凝胶上运行PCR产物,以及检测沙门氏菌。

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