CELL LINE EXPRESSING 5-HT6R IN WHICH HA IS CONNECTED TO N-TERMINAL AND A METHOD FOR DETECTING SEROTONIN 6 RECEPTOR(5-HT6R) LIGAND AT HIGH EFFICIENCY

摘要

PURPOSE: A cell line expressing HA-5-HT6R and a method for detecting serotonin 6 receptor ligand are provided to enhance detection efficiency and to diagnose and treat brain diseases such as depression and Alzheimer.;CONSTITUTION: A cell line which stably expresses HA-5-HT6R is prepared by transducing a vector containing a 5-HT6R gene construct connected with HA(hemagglutinin) to host cells. The cell line is deposited in a deposit number KCLRF-BP-00187. The host cells are HEK293, COS7, CHO-K1 or CHO-G5A. A method for detecting 5-HT6R ligand at high efficiency comprises: a step of treating a test compound to cell line which stably expressing HA-5-HT6R; a step of measuring 5-HT6R activity of treated cell line; and a step of selecting test compound which increases or decreases 5-HT6R activity in the cell line comparing to untreated cell line.;COPYRIGHT KIPO 2010