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PCR PRIMER PAIR DETECTING PEANUT CLUMP VIRUS AND METHOD FOR DETECTING PEANUT CLUMP VIRUS USING THE SAME
PCR PRIMER PAIR DETECTING PEANUT CLUMP VIRUS AND METHOD FOR DETECTING PEANUT CLUMP VIRUS USING THE SAME
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机译:PCR底漆对检测花生菌丛病毒和使用相同方法检测花生菌丛病毒的方法
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摘要
PURPOSE: A PCR primer pair for detecting peanut clump virus(PCV) and a method for detecting PCV using the same are provided to quickly and accurately detect PCV from unknown sample. CONSTITUTION: A PCR primer pair for detecting peanut clump virus(PCV) comprises a forward primer selected from sequence numbers 1-10 and a reverse primer selected from sequence numbers 11 to 18. The PCR primer pair for detecting PCV comprises: a primer pair of sequence numbers 1 and 17, sequence numbers 4 and 14, sequence numbers 4 and 16, sequence numbers 5 and 13, sequence numbers 6 and 15, or sequence numbers 9 and 13. A method for detecting PCV from a sample comprises: a step of isolating RNA from the sample; a step of performing RT-PCR; and a step of separating PCR product by size.
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