Method and kit for expressing protein under regulation of the expression from related sequence formed by gene amplication and transformant

摘要

The present invention provides such a method and kit for releasing the inhibition of transcription due to the repeat sequence of the gene , it establishes a system for the production of protein in large quantities. When the method of the present invention to amplify a gene encoding (a) desired protein, - phage DNA 10kbp or more, such as a polynucleotide, or a method for co-amplifying the insulator sequence, (b) increasing the gene amplification occurs cells () method for selection by culturing in a culture medium containing the drug at a concentration, (c) a method of improving the promoter activity to induce the expression of the gene encoding the target protein, (d) the amplified gene region Cre-LoxP System method is cut out using a chromosome, (e) a gene amplification method is the treatment with 5-aza-2'-cells takes place deoxycytidine (5-aza-2'-deoxycytidine) by reducing the level of methylated DNA , (f) gene amplification is achieved by a double-minute chromosomes (double minute chromosome) at least one or more of the method for selecting the mammalian cells is going on.