Stabilization manner of the assay reagent, at least one biology from the reagent container and its

摘要

A reagent container for detection and / or quantification of chemical analyte or at least one biologically derived from the sample, the reagent container, includes an inner surface surrounding the volume, the present invention is at least 1 volume analytical reactions of one analysis takes place at least for the detection and / or quantification of analytes Tsu, two reagents can be dried in the inner surface of at least the analysis of the subject, the at least one first reagent, spaced apparent from the second region of the inner surface of the second reagent one is dried for at least, that is, a reagent container dried in a region on the first inner surface no overlap whatsoever. Reagent and the second reagent first, an enzyme reagent of the first, is that the second reagent forms a pair which is a substrate for the enzyme, characterized in the reagent container, the pair is It is constituted from the substrate and a nucleic acid polymerase. On the inner surface of the reagent container, also relates to a method of stabilizing a dry assay reagents for the detection and / or quantification of a chemical analyte biological or derived from a sample, the present invention, the method comprising: stages that, to remove excess water from the reagent; that is partitioned two reagents, the reagent and the second reagent of the first at least on the inner surface of the reagent containers is required for the detection of analyte It is to be understood to include, and spaced distinctly from the second region of the inner surface of a second reagent is dispensed thereon, that is, the first reagent includes a first inner surface no overlap whatsoever encompasses be distributed over an area. Reagent and the second reagent first, an enzyme reagent of the first, is that the second reagent forms a pair which is a substrate for the enzyme, characteristics of the method, the nucleic acid polymerase to the pair and is to be composed of the substrate. The invention is further assays utilizing the reverse transcriptase polymerase chain reaction (PCR) assays, reverse transcriptase polymerase chain reaction, immuno PCR assay, a nucleic acid sequence the basic assay (NASBA), proximity ligation assay, a ligase chain reaction ( LCR) assay, a rolling circle amplification (RCA) assay, and the use of the reagent container for performing an assay selected from the group consisting of strand displacement amplification from (SDA) assay.