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Variation of Recombinant Expression Titres By Optimising Bacterial Ribosome Binding Sites
Variation of Recombinant Expression Titres By Optimising Bacterial Ribosome Binding Sites
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机译:通过优化细菌核糖体结合位点的重组表达滴度的变化。
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摘要
The present invention provides a method for optimising the ribosome binding site of a promoter for the expression of a gene encoding a polypeptide of interest, placed under the control of said promoter. The invention also relates to a vector containing such optimised promoters, a prokaryotic host cell transformed by said vector, as well as a method for producing a recombinant protein of interest.
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