首页> 外国专利> Gene fragment coding the genetically modified short of 6 - fosfofruto - 1 kinase, and phosphorylation of proteu00ecna molecule required for the activation, expression vector, bodies, use of this gene as well as procedure for the formation of bioproducts

Gene fragment coding the genetically modified short of 6 - fosfofruto - 1 kinase, and phosphorylation of proteu00ecna molecule required for the activation, expression vector, bodies, use of this gene as well as procedure for the formation of bioproducts

机译:编码经过基因修饰的6-fosfofruto-1激酶的短片段,以及激活,表达载体,抗体,该基因的使用以及形成生物产物的程序所需的蛋白分子的磷酸化

摘要

Gene fragment coding the genetically modified short of 6 ~ fosfofruto - 1 kinase, and phosphorylation of proteu00ecna molecule required for the activation, expression vector, bodies, use of this gene as well as procedure for the formation of bio products.The present invention relates to gene MX - pfka cut modified coding fragment more short of 6 - fosfofruto - 1 - kinase (pfki) without the need for phosphorylation of the protein molecule for the activation, which is not significantly inhibited by citric acid and / or their salts and molecules. ATP.The fragment of 49 to 52 kDa active coded by the gene MT pfka retains the positive properties of the regulatory natural protein and is activated in the presence of specific triggers, while citric acid and ATP are important metabolites that function as feedback inhibitors in S higher organisms, does not reduce its activity.The invention deals with the use of the fragment more short changed in biotechnological processes for producing primary and secondary metabolites.
机译:编码短链6〜fosfofruto-1激酶的基因修饰的基因片段,以及激活,表达载体,体,该基因的用途以及形成生物产物的程序所需的蛋白分子的磷酸化。涉及基因MX-pfka切割的修饰的编码片段,比6-fosfofruto-1-激酶(pfki)更短,而无需将蛋白质分子磷酸化以进行激活,而柠檬酸和/或它们的盐不会明显抑制该分子的活化和分子。由MT pfka基因编码的49至52 kDa活性片段保留了调节性天然蛋白的阳性特性,并在特定触发条件下被激活,而柠檬酸和ATP是重要的代谢产物,在S的反馈抑制剂中起作用本发明涉及使用在生物技术过程中变化更短的片段来生产初级和次级代谢产物的用途。

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