method of selection of individual therapeutic dose of gaseous biologically active substances

摘要

A method of selection of individual therapeutic dose of gaseous biologically active substances comprises the indirect assessment of the extent of damage of nativity of blood cells membranes. As cells the blood lymphocytes are selected, isolated to the density gradient from heparinized blood of the patient's physiological solution is saturated with various doses of the active gas substance and added to a certain number of serum samples series of the same patient. Then the mixture is incubated with isolated lymphocytes, lymphotoxicity is assessed and the dose of ozone is determined, as recommended to use by this patient.