Mycobacterium avium strain type II supespecies for tuberculosis and applications.

摘要

Mycobacterium avium paratuberculosis strain subspecies type II and applications.; The present invention relates to a new strain of Mycobacterium avium subspecies paratuberculosis has suffered a deletion of 16.3 Kb for mce lacking genes (mammalian cell entry). Furthermore it relates to a method developed based on the polymerase chain reaction to detect other strains of Mycobacterium avium subspecies paratuberculosis lacking these genes. Furthermore, in the present invention validation methodologies for the use of this strain of Mycobacterium avium subspecies paratuberculosis CECT 7530 in assays in vitro invasion in different cell lines of diverse origins it reflected. Additionally, the present invention mentions the development of methods for measuring the viability of Mycobacterium avium subspecies paratuberculosis within eukaryotic cells during the process of in vitro invasion by estimating the number of copies of precursor 16S rRNA gene pre -ARNr 16S.