Generating data files flow cytometry with a potentially infinite number of dimensions derived from the fusion of a group of data files separate flow cytometry and multidimensional reconstruction both flow cytometry data actually measured and estimated.

摘要

A method for generating new data files flow cytometry from original data files containing parameter data about events measured in different aliquots of the same sample in each file of original data comprises data common parameters corresponding to parameters measured for all aliquots and data not common parameters corresponding to parameters measured only for some aliquots, the procedure comprising the step of: a) fusing two or more original data files in one file; characterized by further comprising the steps of b) for each event merged file estimate data of non-common parameters that were not directly measured in the flow cytometer based on events pertaining to aliquots for which said non-common parameter has It was measured by flow cytometer and a corresponding uncertainty measure, and; c) reconstructing a new data file containing data measured data parameters and parameter estimates.