IMPROVED METHOD FOR DETECTION OF SPECIFIC TARGET CELLS IN SPECIALIZED OR MIXED CELL POPULATION AND SOLUTIONS CONTAINING MIXED CELL POPULATIONS

摘要

The invention relates to a method for detecting specific target-cells in asimple and time saving way, using paramagneticparticles, antibodies recognizing the Fc portions of target-cell associatingantibodies and target-cell associating antibodiesdirect-ed to specific antigen determinants in the target-cell membranes.Incubation of the cell suspension with a mild detergent and/orsecond set of antibodies or antibody fragments, prelabeled or not withfluorescent agents, metallocolloids, radioisotopes,biotin--complexes or certain enzymes allowing visualization, will dramaticallyincrease the specificity of the method. The method canfur-ther be used for isolation of the target-cells by magnetic fieldapplication and a kit for performing the method according to theinvention is described.