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Probes for genotyping low-risk-HPV

机译:低风险HPV基因分型的探针

摘要

The current invention is concerned with a composition comprising at least one probe oligonucleotide each for the nucleotide sequences of the invention, said probe oligonucleotides specifically hybridizing to the sense strand or the antisense strand of said nucleotide sequences. Moreover, the present invention relates to a method for the identification of low-risk HPV types in a sample comprising the steps of a) contacting a sample with an amplification composition allowing amplification of at least one region of the HPV genome specifically hybridizing to at least one of the probe oligonucleotides of the current invention under conditions which allow for the amplification of polynucleotides and b) identifying low-risk HPV genotypes in said sample based on the amplified polynucleotides obtained in step a) by hybridizing the amplified polynucleotides with at least one labelled probe oligonucleotide of the current invention while said amplified polynucleotides are present in the same reaction container. The invention also relates to the use of a composition of the current invention for identifying low-risk HPV in a sample and to a kit comprising the composition of the invention and / or adopted for carrying out the method of the invention and an instruction manual.
机译:本发明涉及一种组合物,其包含至少一个各自用于本发明核苷酸序列的探针寡核苷酸,所述探针寡核苷酸与所述核苷酸序列的有义链或反义链特异性杂交。而且,本发明涉及一种用于鉴定样品中低风险HPV类型的方法,其包括以下步骤:a)使样品与扩增组合物接触,所述扩增组合物允许扩增HPV基因组的至少一个区域,所述区域至少与至少一种杂交。在允许扩增多核苷酸的条件下,本发明的探针寡核苷酸之一;和b)基于在步骤a)中获得的扩增的多核苷酸,通过将扩增的多核苷酸与至少一种标记的杂交来鉴定所述样品中的低风险HPV基因型当所述扩增的多核苷酸存在于相同的反应容器中时,本发明的探针寡聚核苷酸。本发明还涉及本发明的组合物在鉴定样品中的低风险HPV中的用途,并且涉及包含本发明的组合物和/或用于实施本发明的方法和说明书的试剂盒。

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