A DNA MICROARRAY WITHOUT CROSS-TALK SEQUENCES FOR FOOD-BORNE PATHOGEN DETECTION

摘要

The present invention relates to a DNA microarray for detecting microbial causes of food poisoning, and more specifically, includes a random DNA probe derived from genomic DNA of a microbial causative organism to be detected, but cross-talk hybridization with a comparative food microbial microorganism. The DNA probe capable of causing hybridization provides a DNA microarray for detecting the cause of food poisoning bacteria, which is excluded. According to the present invention, a DNA microarray for detecting food poisoning causes microorganisms may be prepared using a random probe derived from a food poisoning cause microorganism to be detected. The DNA microarray for detecting food poisoning causes microorganisms can detect food poisoning causes microorganisms without accurate sequence information of food poisoning and food decay-causing microorganisms, thereby reducing the cost and time required to obtain sequencing information, and using some of the probes. There is an effect of reducing the detection failure rate due to the cross-talk reaction that can appear. In addition, since the simultaneous diagnosis of various food poisoning bacteria provides more accurate information about the contaminating microorganism than the microorganism detection using the indicator microorganism, it is expected to establish a fundamental solution than the problem occurrence and ultimately contribute to the improvement of public health.