METHOD OF MICROBIOLOGICAL SYNTHESIS OF MATURE HUMAN INTERFERON ALPHA-2, Saccharomyces cerevisiae STRAIN - MATURE HUMAN INTERFERON ALPHA-2 PRODUCER (VERSIONS)

摘要

FIELD: medicine.;SUBSTANCE: method of microbiological synthesis of mature human interferon alpha-2 is implemented by cultivation of Saccharomyces cerevisiae yeast containing inactivating mutation in a structural gene of proteinase YPS1 and/or additional genes of proteinase KEX2. A recombinant method is used to produce Saccharomyces cerevisiae yeast strains capable to secrete mature human interferon alpha-2 in a culture medium.;EFFECT: invention allows increasing production of mature human interferon alpha-2 ensured by reduced degradation of secreted interferon by yeast proteinase YPS1 gene inactivation, and by improved effectiveness of processing of secreted interferon precursor by increasing native or secreted proteinase KEX2 expression.;9 cl, 1 tbl, 33 ex