CULTURE MEDIUM FOR THE DETECTION OF MICROORGANISMS BY FLUORESCENCE ALLIING A FLUOROGENEOUS SUBSTRATE AND A PH-SENSITIVE FLUOROPHORE

摘要

The invention relates to a culture medium that combines a fluorogenic substrate and a pH-sensitive fluorophore, in particular the combination of 4-methylumbelliferone (4-MU) and fluorescein derivatives. This culture medium is used for the fluorescence detection of microorganisms by coupling relative fluorescence measurements, on the one hand, to the pH-sensitive fluorophore, and on the other hand to the activation by the microorganisms of the substrate (s) (s). ) fluorogenic (s).