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Urine pretreatment agent for urinary protein quantitation, urine pretreatment method, and urinary protein quantitation method

机译:用于尿蛋白定量的尿预处理剂,尿预处理方法和尿蛋白定量方法

摘要

Described are a urine pretreatment agent, a urine pretreatment method, and a urinary protein quantitation method which reduce or cancel the influences of urine pH variations, cancel the influences of precipitates of urinary inorganic salts, and solubilize membrane proteins. The urine pretreatment agent for urinary protein quantitation, includes a buffer, a chelating agent, and a surfactant; the urine pretreatment method includes a step of mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and the urinary protein quantitation method includes steps of: mixing 10 to 1000 parts by mass of the urine pretreatment agent with 100 parts by mass of urine; and then measuring the protein concentration.
机译:描述了一种尿预处理剂,一种尿预处理方法和一种尿蛋白定量方法,其减少或消除了尿液pH变化的影响,消除了尿无机盐沉淀的影响,并使膜蛋白增溶。用于尿蛋白定量的尿液预处理剂,包括缓冲剂,螯合剂和表面活性剂。尿液预处理方法包括将10至1000质量份本发明的尿液预处理剂与100质量份尿液混合的步骤;尿蛋白定量方法包括以下步骤:将10至1000质量份的尿液预处理剂与100质量份的尿液混合;以及然后测量蛋白质浓度。

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