Based bioformulado biofungicide for the control of Bacillus amyloliquefaciens fumonisins in Maize Harvest, a procedure to improve the efficiency of the formulated biofungicide and a preparation method thereof and Application

摘要

It also describes methods for mass production in the fermenter, packaging and formulation to optimize Efficiency, Conservation and preparation of the formulated product, From This strain, Time and method of application. This has application in the agro Food Sector and especially in Plant Protection of maize Harvest.Claim 1: Based bioformulado biofungicide Bacillus amyloliquefaciens to be applied as seed in the control of Income verticillioidesen Fusarium in Maize plants or Grains, particularly Corn Zea mays L.Characterized by containing paraffin oil 2% compound as Facilitator of the adhesion on the surface of the Grain of Corn used as SEED, and biocontrol bacterial Strain identified as Bacillus amyloliquefaciens, which has the following characteristics morfofisiolog Characteristics and Genetic or Bacillus Spore Gram (+), aerobic, catalase, Voges Proskauer ((+) - +)Tryptophan Aminopeptidase (+), hydrolysis of STARCH (+), Mobile peru00edtrica Flogging or far; 16S sequences were amplified with the primers FD1 and RD1 following the program initial denaturation (94u00b0C 3 Cycles of amplification (33 ') 45u00f6 to 94u00b0C, 1' and 2 '56u00b0C 72u00b0C) and a Step End (5 'extension 72u00b0C); and the Amplification products were sequenced by Macrogen Inc.Korea; and the obtained sequences were compared with the database of the National Center for Biotechnology Information (NCBI)Or National Center for Biotechnology Information) using an analysis of blastn and assigned its Taxonomic Affiliation on the basis of the maximum percentages of identity and 16 s rDNA sequences have been deposited in the NCBI Database (GenBank Access eu164542 or Strain; Biofungicide bacterial infectious dose has an average of 106 - 107 Colony Forming UnitsMl (CFU.ML - 1) against Fusarium verticillioides, or is compatible with fungicides and insecticides Selected Chemicals, Vitavax Flo, at least:Fludioxinil + metalaxyl and insecticide clothianidin; or its application does not affect the native microflora rizosfu00e9rica and not rizosfu00e9rica; or its application does not affect the index of diversity of culturable microorganisms and does not affect the diversity indices obtained by molecular techniques. Or your application does not affect Soil microbial processes; or is not pathogenic or toxic for plants,Animal or human cell Survival; or on the surface of Corn Seeds, is of the Order of 103 - 105 cfu ml - 1 after inoculation with a level of 109 CFU ml - 1 to 30 days pelleteado; or can be maintained and preserved long-term (12 months or Lyophilized); maintaining And keep short-term Nutrient Agar 2 - 4oC or frozen at - 80 degrees centigrade and / or glycerol - 20 to 30% w / V.