DNA constructs for activation and modification of endogenous gene expression

摘要

NORMALLY TRANSCRIPTIONALLY INACTIVE GENES IN A EUCARIOTIC CELL LINE CAN BE ACTIVATED FOR EXPRESSION THROUGH THE INSERTION OF A DNA REGULATORY ELEMENT THAT IS ABLE TO PROMOTE THE EXPRESSION OF A GENETIC PRODUCT NORMALLY EXPRESSED IN THAT CELLULATED INSTITUTE. TO THE NORMALLY INACTIVE GEN IN QUESTION. THE INSERTION IS CARRIED OUT BY HOMOLOGICAL RECOMBINATION, USING A DNA CONSTRUCTION THAT INCLUDES A NORMALLY INACTIVE GENE DNA SEGMENT ("DIANA DNA") AND THE DNA REGULATORY ELEMENT TO INDICATE TRANSCRIPTION. THE TECHNIQUE IS ALSO USED TO MODIFY THE EXPRESSION CHARACTERISTICS OF ANY ENDOGENOUS GENE OF A GIVEN EUCARIOTIC CELL LINE.