PROCEDURE FOR THE DETECTION OF HIGH VIRUS CONCENTRATIONS IN BLOOD PLASMA AND/OR BLOOD SERUM BY MEANS OF THE POLYMERASE CHAIN REACTION

摘要

A procedure for the detection of high virus concentrations in blood plasma and/or blood serum by means of the polymerase chain reaction (PCR) is described, in which the sensitivity of the PCR is restricted by the use of suboptimal nucleic acid extraction, amplification or detection conditions. This procedure is suitable, for example, for the detection of parvovirus DNA in plasma or serum, it being possible to adjust the sensitivity of the PCR such that the parvovirus DNA is only detected in samples whose DNA content is greater than 10 6 to 10 7 genome equivalents/ml. The detection of the parvovirus DNA amplification carried out is carried out in the sample by measurements of the fluorescence.