METHOD OF PRODUCING AN UNDIFFERENTIATED AVIAN CELL CULTURE USING AVIAN PRIMORDIAL GERM CELLS

摘要

A method of producing undifferentiated avian cells expressing an embryonicstem cell phenotype. The method includes the steps of collecting avian gonadalcells comprising primordial germ cells from an avian embryo after theformation of the primitive streak; depositing the avian gonadal cells incontact with a preconditioned feeder matrix; and growing the avian gonadalcells on the pre-conditioned feeder matrix in the presence of media for a timesufficient to produce an avian cell culture consisting essentially ofundifferentiated avian cells expressing an embryonic stem cell phenotype.