ANTIBODY FOR ASSAY OF ADAMTS13 ACTIVITY AND METHOD FOR ASSAYING THE ACTIVITY

摘要

It is intended to provide an antibody, especially monoclonal antibody, usefulfor the determination of ADAMTS13 activity, and provide a method ofdetermining the activity of ADAMTS13. Further, it is intended to provide amonoclonal antibody that exhibits specific reactivity to any antigendeterminant site occurring when ADAMTS13 is caused to act on a partial peptideof VWF able to become a substrate or VWF as a substrate but has no specificreactivity to complete VWF molecules, and to provide a usage of the antibody.These have been achieved by success in obtaining of a monoclonal antibody(anti-N-10 monoclonal antibody) having specific reactivity to cleavage site atwhich a partial peptide of VWF is specifically cut by ADAMTS13 and by findingof a method of determining the ADAMTS13 activity by the use of this monoclonalantibody.