Method for preparing evolved micro-organisms, enabling the creation or modification of metabolic pathways

摘要

the present invention relates to a process for preparing advanced microorganisms, allowing the modification of metabolic pathways using nadph, characterized in that it comprises the following steps:(a) genetic modification of the cells of an organism, so that the initial rate of production of nadph oxidation rate is higher than its in nadp, microorganism, consisting of one or several genes encoding enzymes involved in the biotransfo nadph dependent s of a molecule of interest.(b) culture of microorganisms changed previously obtained on a defined medium to bring such genes coding for the nadph dependent enzymes involved in the biotransformation of a molecule of interest.(c) the selection of cells of microorganisms capable of growing on the modified defined medium.;the invention relates to a microorganism changed can be obtained by the process described above, as well as its use in a process of biotransformation consuming nadph.