Vaccine composition for protection and treatment against pathogenic Escherichia coli and Salmonella in cattle by administration of attenuated Salmonella expressing adhesin gene of pathogenic Escherichia coli and vaccination method thereof

摘要

The present invention provides Salmonella mutants which comprise an adhesin gene of bovine pathogenic Escherichia coli, and are transformed into attenuated Salmonella mutants wherein asd, lon and cpxR genes are deleted; and a vaccine composition for preventing and treating bovine Colibacillosis and Salmonellosis comprising the same. The Salmonella mutants of the present invention efficiently deliver an antigen derived from Escherichia coli into bovine mucosa lymphoid tissues and induce powerful mucosal immunity, and enable the production of the associated vaccine and mass innoculation. In addition, the Salmonella mutants of the present invention allow the innoculation cost and the side effects such as pain, inflammation or the like to be reduced. The production and storage of the Salmonella mutants of the present invention are easy and thus economical. Therefore, the Salmonella mutants of the present invention prevent infections by inducing immune responses against Salmonella and can be used as a polyvalent vaccine due to the inducement of immune responses against expressing exogenous antigens.