OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS B. pseudomallei AND B. mallei

摘要

FIELD: medicine.;SUBSTANCE: described is oligonucleotide probe with complementary end sequences by type of "molecular beacon", ensuring fluorescent detection in identification of causative agents of glanders and melioidosis B. pseudomallei and B. Mallei by method of polymerase chain reaction with oligonucleotide primers BTTS-sl/BTTS-as3, complementary to fragment of gene 23S pRNA B. pseudomallei and B. mallei: 5'-(FAM)-CGCGCACCGGCAGTGATGAGCCACGCGCG-(RTQ1)-3', where FAM is carboxyfluorescein, fluorescent dye, whose absorption wavelength constitutes 492 nm, and fluorescence wavelength is 520 nm, RTQ1 is fluorescence quencher with range of quenching 470-570 nm.;EFFECT: application of hybridisation probe makes it possible to identify causative agents of glanders and melioidosis in short term with high sensitivity and specificity in biological material and environment objects.;3 ex