METHOD AND DEVICE FOR CARRYING OUT A QUANTITATIVE SPATIALLY RESOLVED LOCAL ANALYSIS AND DISTRIBUTION ANALYSIS OF CHEMICAL ELEMENTS AND IN SITU CHARACTERIZATION OF ABLATED SURFACE REGIONS

摘要

A laser ablation chamber, which is suitable for use in a conventional laser-assisted micro dissection unit (LMD), in combination with the LMD allows for both quantitative spatially resolved nanolocal analysis and distribution analysis of element concentrations of a sample, and a microscopic detection of the surface topography of the same sample, in the nanometer range. Optionally, further examinations may follow, without having to remove the sample from a microscope slide bearing the sample. For the examination, a region of the sample to be analyzed is selected with the aid of a microscope of a LMD. For this purpose, the sample is located on the lower face of a cover slip (microscope slide), which also forms part of a laser ablation chamber mounted beneath the microscope slide and inside the LMD. A portion of the sample is ablated and analyzed. Optionally, the existing LMD instrumentation may be used to deliberately cut out certain regions of the tissue in which metals were detected for further analytics and to collect these regions in sample containers, which are mounted beneath the microscope slide after the laser ablation.