Method for controlling biooxidation reactions

摘要

A method for controlling a biooxidation reaction comprising the steps of: (1) adding formaindependiente a substrate selected from the group consisting of a carboxylic acid or an alkane and uncosustrato at predetermined rates to a reaction mixture of biooxidation that how much a biocatalizadorque is Candida tropicalis H5343, (2) measuring the rate of oxygen uptake and release rate dedióxido carbon of reaction mixture, (3) determining the instantaneous rates of consumption sustratoy cosubstrate by of solving simultaneous equations concerning the release rate dedióxido carbon and the rate of oxygen consumption with respect to the stoichiometry of substrate oxidation, laestequiometría combustion cosubstrate, and optionally stoichiometry biomass formation ; (4) simultaneously adjusting the rate of addition of substrate and cosubstrate regarding rates delsustrato oxidation and consumption cosubstrate in order to maximize the rate of product formation while that WILL minimizes the use rate of cosubstrate .