Method for measuring plasmin activity of microparticles in a sample of a biological fluid and use thereof

摘要

Method for the measurement of plasmin activity in isolated microparticles, particularly circulating lasmicropartículas of a blood sample, previously extracted, in which - in a first step said microparticles present in said sample are isolated, according SomeProcedure in which or at a stage 1A a volume of between 500 .mu.l and 5 ml of a bleed sample previously extracted at a speed between 1000 g and 2000 g, during understood untiempo between 5 minutes and 20 minutes, at a temperature between 2 centrifuged y6ºC ; or at a stage 1B the supernatant obtained in step 1A to velocidadcomprendida between 10000 g 20000 g, for a time between 1 minute and 5minutes at a temperature between 2 and 6 ° C and centrifuged; or at a stage 1C the supernatant obtained in step 1B to velocidadcomprendida between 15000 g 25000 g centrifuged for a time between 45 minutes 120minutos, at a temperature between 2 and 6 ° C; or a 1D step the pellet obtained in step 1C in comprendidoentre volume 250 ul and 4 ml of phosphate buffered saline (PBS) buffer is collected and the mixture to a velocidadcomprendida between 15000 g 25000 g centrifuged for a time of between 45 120minutos minutes, at a temperature between 2 and 6 ° C; or a 1E step the pellet obtained in step 1D in comprendidoentre volume 250 ul and 4 ml of phosphate buffered saline (PBS) is collected and the mixture to a velocidadcomprendida between 15000 g 25000 g centrifuged for a time between 45 120minutos minutes, at a temperature between 2 and 6 ° C; 1F or in a stage the clot obtained in step 1E comprendidoentre in a volume 20 ul and 500 ul of phosphate buffered saline (PBS) is collected. - in a second stage is measured by any appropriate method, the ability of such micropartículasaisladas in step 1 to generate plasmin, and - in a third step the measurement result obtained in step 2 with the result unamedida identical compared performed under the same conditions in a sample of blood identical witness.