A METHOD FOR PREPARING HBV VACCINE COMPRISING ALUMINUM ADJUVANT

摘要

The present invention discloses a method for preparing HBV vaccine comprising Aluminum adjuvant, which belongs to biological technology field. The method, which is characterized in that aluminum adjuvant Al(OH)3 is produced by an on-line reaction, comprises mixing PBS buffer solution and potassium aluminum sulfate (KAl(SO4)2) solution with hepatitis B surface antigen stock solution, adding sodium hydroxide(NaOH) solution into the mixed solution, so that the adjuvant is continuously produced and hepatitis B surface antigens are continuously coated and adsorbed simultaneously. The process is called "in-situ adsorption".