FUNCTIONAL ENHANCEMENT OF YEAST TO MINIMIZE PRODUCTION OF ETHYL CARBAMATE VIA MODIFIED TRANSPORTER EXPRESSION

摘要

The invention provides a Saccharomyces cerevisiae strain that is transformed to constitutively express DUR3, encoding a urea transporter protein, under the control of the phosphoglycerate kinase (PGK1) promoter and terminator sequences, resulting in reduced nitrogen catabolite repression, wherein said transformed yeast strain may be further transformed to constitutively express a urea degradation enzyme, such as urea carboxylase-allophanate hydrolase or urea amidolyase, also resulting in reduced nitrogen catabolite repression, a method for generating said strain, and a method and use of said strain to produce a fermented beverage or food product with a reduced ethyl carbamate concentration of less than 30 ppb.