Disclosed in the present invention are a selenium-rich Cardamine Hupingshanensis plant material and the cultivation method thereof; selenium is present in the form of selenocysteine in the plant material, and the selenium content in the form of selenocysteine in the plant material is 0.2 mg/kg-20000 mg/kg, accounting for above 90% of the total content of selenium. The selenium-rich Cardamine Hupingshanensis plant material is cultivated according to conventional methods; the selenium source is added to the growth medium of Cardamine Hupingshanensis within the growth cycle of Cardamine Hupingshanensis, with the selenium content of the growth medium adjusted to 0.1-1000 mg/kg, and meanwhile a basic substance is added to keep the pH value of the whole or a local environment of the growth medium within the range of 7-8. The material can be ingested directly as a better selenium-sourced supplemental substance for the human body and animals, or can be used as a selenium-rich food or selenium-rich feed, which are beneficial for human and animal health.
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