CANCER-SPECIFIC GENE THERAPEUTIC AGENT BY REGULATING EXPRESSION USING miRNA

摘要

PURPOSE: A cancer-specific gene therapeutic agent using microRNA is provided to regulate expression of an expression vector during post-transcription and to reduce side effects.;CONSTITUTION: An expression vector contains a promoter, a trans-splicing ribozyme coding DNA which is operatively linked with the promoter and acts in a cancer-specific gene; and a microRNA target sequence coding DNA. The expression vector additionally contains a therapeutic gene or reporter gene. The cancer-specific gene is hTERT(human telomerase reverse transcriptase). The ribozyme is trans-splicing group I intron ribozyme which specifically targets hTERT mRNA. The reporter gene is firefly luciferase gene or renillar luciferase. The promoter is a promoter of PEPCK(phosphoenolpyruvate carboxykinase) gene. The expression vector contains a base sequence selected from sequence numbers 4-9. A pharmaceutical composition for anti-cancer contains the expression vector as an active ingredient.;COPYRIGHT KIPO 2013