METHOD FOR DETECTING A CONTAMINANT FROM A SAMPLE BY QUANTUM DOT-BASED COMPETITIVE IMMUNOASSAY AND MULTIPLEX FLOW CYTOMETRY

摘要

PURPOSE: A method for detecting a contaminant from a sample by quantum dot-based competitive immunoassay and multiplex flow cytometry is provided to enhance sensitivity (reduction of LOD and increase of measurement range), to shorten analysis time, and to simplify operation, thereby quickly and easily diagnosing dangerous factors in the sample with high sensitivity.;CONSTITUTION: A method for detecting a contaminant from a sample by quantum dot-based competitive immunoassay and multiplex flow cytometry comprises the steps of: preparing polystyrene bead-antigen competitive complexes in which each antigen is conjugated to polystyrene beads; preparing quantum dot probes in which each antibody is conjugated to quantum dots; applying the polystyrene bead-antigen competitive complexes to a sample solution to be analyzed; and adding the quantum dot probes; and analyzing the sample solution using a flow cytometry device.;COPYRIGHT KIPO 2013;[Reference numerals] (1) Produce a sample solution; (2) Add polystyrene bead-antigen (PB/Ag) competitive complexes; (3) Add quantum dot detecting probe; (4) Competitive immune reaction; (5) Implement a flow cytometry; (AA) Pollutant (antigen) 1; (BB) Pollutant (antigen) 2; (CC) PB/Ag complex 1; (DD) PB/Ag complex 2; (EE) Quantum dot detecting probe 1; (FF) Quantum dot detecting probe 2; (GG) Quantum dot detecting probe combined PB/Ag complex; (HH) Laser; (II) Photoelectron multiplier (fluorescence channel 1); (JJ) Photoelectron multiplier (fluorescence channel 3); (KK) Collect data; (SS) Side Wall Scattering