首页> 外国专利> A Paenibacillus sp.HPL-003 strain for producing xylanase and an thermostable,wide pH spectrum,and highly active xylanase produced thereby and a transgenic mass-producing method thereof

A Paenibacillus sp.HPL-003 strain for producing xylanase and an thermostable,wide pH spectrum,and highly active xylanase produced thereby and a transgenic mass-producing method thereof

机译:一种产木聚糖酶的Paenibacillus sp.HPL-003菌株及其产生的热稳定,宽pH范围和高活性木聚糖酶及其转基因批量生产方法

摘要

PURPOSE: A novel protein isolated from novel Penibacillus sp. is provided to develop biopolymers, foods, and feed. CONSTITUTION: A xylanase has an amino acid sequence of sequence number 4. A strain producing xylanase is Paenibacillus sp. HPL-003(deposit number KCTC 18179P). A polynucleotide encoding the xylanase has a base sequence of sequence number 3. A recombinant expression vector contains the polynucleotide encoding xylanase. A method for producing the xylanase comprises: a step of introducing the recombinant expression vector to a host cell to prepare a tranformant; a step of culturing the transformant; and a step of centrifuging to collect crude enzyme liquid.
机译:目的:从新型Penibacillus sp。中分离出一种新型蛋白质。提供用于开发生物聚合物,食品和饲料。组成:木聚糖酶具有序列号4的氨基酸序列。产生木聚糖酶的菌株是Paenibacillus sp。 HPL-003(保藏号KCTC 18179P)。编码木聚糖酶的多核苷酸具有序列号3的碱基序列。重组表达载体包含编码木聚糖酶的多核苷酸。木聚糖酶的生产方法包括:将重组表达载体导入宿主细胞以制备转化子的步骤;培养转化体的步骤;离心分离粗酶液的步骤。

著录项

  • 公开/公告号KR101219518B1

    专利类型

  • 公开/公告日2013-01-11

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20100061341

  • 发明设计人 황인택;박노중;임희경;

    申请日2010-06-28

  • 分类号C12N9/14;C12N15/55;A23K1/16;C12P7/04;

  • 国家 KR

  • 入库时间 2022-08-21 16:25:57

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