PURPOSE: A method for preparing arabinose from hemicellulose arabinan is provided to produce functional monosaccharides. CONSTITUTION: A method for preparing L-arabinose comprises a step of immobilizing endo-1,5-alpha-L-arabinanase and alpha-L-arabinofuranosidase to a carrier. A carrier for immobilization is duolite A568. The alpha-L-arabinofuranosidase has an amino acid sequence of sequence number 1. The endo-1,5-alpha-L-arabinanase has an amino acid sequence of sequence number 2. A complex is prepared by immobilizing the enzyme to the carrier. A composition for producing L-arabinose contains the complex.
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