DEVICE FOR VERTICAL electrophoresis of nucleic acids and proteins in GRADIENT GELS

摘要

The utility model is intended for vertical electrophoresis of biomolecules and analytical equipment belongs to a new generation has the capacity scalability and modularity, including the possibility of connecting additional blocks of reading and / or course of the electrophoresis process of data transfer to a computer for subsequent mathematical analysis using standard computer programs. Additional features provide the following field application device, it is necessary to specify an efficient system potting gels with varying porosity and varying linearly denaturant concentration, solid parsed by the housing, original device for fixing gels in the device, temperature control of electrophoretic buffer. The whole complex of the foregoing features provides a substantial expansion of the range of areas of application of the device, increases its efficiency, resolution, productivity. In particular, the one-piece body reduces the time of preparation of the device to work and work stoppage. A simple and more effective system gels fixation device enables the division of the large number of simultaneously used gels greatly simplifies application of samples to the gel, already mounted in the device. Tempering buffer, which is fully submerged gel electrophoresis, allows to separate DNA fragments of identical length but differing in the base sequences of residues that dramatically expands the range of applications of the instrument for the study of gene polymorphism and genomes, as well as for studies of population genetics. The device is designed for a wide range of analytical procedures for the separation of proteins and nucleic acids, including single nucleotide fragment and analysis of gene polymorphism, DNA profiling, RAPD PCR-analysis, DNA-denaturing DGGE analysis, electrophoresis of proteins under native and denaturing conditions, immunochemical analysis of proteins. The goal is maximum universality, significantly simplifying the design and reducing the time of its preparation for use. The simplicity of the device is the lack of hard and tightly connected and complex custom components. The device consists of a container with removable lid containing the electrodes, the capacity is divided into two chambers with a partition with a space connecting the cathode and anode of the buffer in the lower part of the device and the heating element in the same part. Gels are pressed against the wall with the cathodic part cameras by a simple pressing device.