HIGH-CELL DENSITY FED-BATCH FERMENTATION PROCESS FOR PRODUCING RECOMBINANT PROTEINS

摘要

PROBLEM TO BE SOLVED: To provide a high-cell density fed-batch fermentation process for producing recombinant proteins.SOLUTION: Proteins, for example, recombinant meningococcal 2086 proteins, are produced using a fed-batch fermentation process with continuous feeding of an inducer during induction in a culture medium, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields.