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New revelation for various protein generation with colon bacillus and secretion vector system

机译:结肠杆菌和分泌载体系统对各种蛋白质生成的新启示

摘要

As for this invention, with the colon bacillus (E. coli) as for aforementioned system, in order furthermore to help the secretion to the cell outside gap, it is the combination capacity of the transposition which is based on the signal peptide of the rearrangement body protein to the periplasm gap of the colon bacillus, to the imperfect film mutation body of the colon bacillus in regard to recombinant DNA revelation/secretion system. This invention in order to promote the periplasm secretion where the rearrangement body protein which is revealed excessively improves, furthermore regards the revelation system which furthermore includes the helper plasmid in order to promote the revelation of toransurokon. Furthermore, this system promotes also the efficient generation of the protein of specification of the target with the colon bacillus.
机译:对于本发明,对于上述系统,利用结肠杆菌(E.coli),为了进一步帮助向间隙外的细胞分泌,转座的结合能力是基于重排的信号肽的。在重组DNA展示/分泌系统方面,人体蛋白质与结肠杆菌周质间隙,结肠膜的不完美膜突变体有关。为了促进揭示出的重排体蛋白过度改善的周质分泌,本发明还考虑了进一步包括辅助质粒的揭示系统,以促进对呋喃呋喃酮的揭示。此外,该系统还促进结肠芽孢杆菌有效地产生靶标蛋白。

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