METHOD FOR RAPID ISOLATION OF GENOMIC DNA (DEOXYRIBONUCLEIC ACID)

摘要

The present invention relates to a method that has been designed in a rapid kit form for rapid isolation of nucleic acids, preferably genomic DNA from biological samples like blood, buccal cells and tissue samples, comprising the steps of lysing the samples using lysis buffer that acts as a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactants ions from the lysate and separating the deoxyribonucleic acid(DNA) from the precipitate by protein precipitation. As per the invention, the simple lysis of cells with proteinase K without the need for any mechanical lysis has been done. Further processing using convenient silica spin column procedure enables very quick purifications of high quality and ready to use DNA within a time of less than one hour.