首页> 外国专利> PROCESS FOR PRODUCING A PLANTING MATERIAL FREE OF PNRSV BY IN VITRO CULTURES IN SOUR CHERRY TREE VARIETY NAMED SCHATTENMORELLE

PROCESS FOR PRODUCING A PLANTING MATERIAL FREE OF PNRSV BY IN VITRO CULTURES IN SOUR CHERRY TREE VARIETY NAMED SCHATTENMORELLE

机译:通过体外培养的樱桃树变种樱桃树生产不含PNRSV的种植材料的方法

摘要

The present invention relates to a process for producing a planting material free of PRNSV by in vitro cultures in sour cherry tree variety named Schattenmorelle, wherein a biological material consisting of a meristem of a dimension of 0.1...0.2 mm, obtained from buds located on annual branchlets, is cultivated on a growth medium for the differentiation phase, said growth medium comprising the Murashige-Skoog components in the following amounts: 80...110 ml/l from the stock solution of macro-elements with a concentration of 10x, 8...12 ml/l from the stock solution of micro-elements with a concentration of 100x, 8...12 ml/l from the stock solution of vitamins with a concentration of 100x and a hormonal balance represented by 0.05...0.2 mg/l of gibberellic acid, 0.01...0.03 mg/l of indolylbutyric acid and 0.10.5 mg/l of 6-benzylaminopurine.
机译:本发明涉及通过在名为Schattenmorelle的酸樱桃树品种中通过体外培养来生产不含PRNSV的种植材料的方法,其中由尺寸为0.1 ... 0.2mm的分生组织组成的生物材料是从定位的芽中获得的。在一年生小枝上,在分化阶段的生长培养基上进行培养,所述生长培养基包含以下含量的Murashige-Skoog组分:80 ... 110 ml / l的大型元素储备液,浓度为10x ,从浓度为100x的微量元素储备溶液中提取8 ... 12 ml / l,从浓度为100x的维生素储备溶液中提取8 ... 12 ml / l,荷尔蒙平衡为0.05。 ..0.2 mg / l的赤霉素,0.01 ... 0.03 mg / l的吲哚基丁酸和0.10.5 mg / l的6-苄基氨基嘌呤。

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