STANDARD METHOD FOR EXPERIMENTAL EVALUATION OF SIDE TOXICITY OF THERAPEUTIC AGENTS

摘要

The invention relates to standard method for experimental evaluation of side toxicity of therapeutic agents and can be used in medicine, in particular in oncology, pharmacology and toxicology. According to the method, in the lysates of the organs under investigation a DNA content and viscosity are determined, after that the coefficient of toxicity (CT) of therapeutic agent with respect to specific organ is calculated by formula,where m – weight of the tissue sample; V – volume of lysate obtained; Δt – difference between the time of flow through viscosimeter of unit volume of lysate after leveling concentrations of DNA in lysates and time of flow of pure lytic solution through viscosimeter; C– concentration of DNA in initial lysate or any value that is directly proportional to concentration of DNA, for instance extinction of outlet lysate at wavelength of radiation 260 nm, and the results of calculations obtained are interpreted as follows: if CT1 – side toxicity of therapeutic agent as to that organ is present, and toxicity is larger with larger value of CT; if the CT1 – therapeutic agent promotes survival of the organ cells. The invention makes it possible to determine organo-specific side toxicity of therapeutic agent much more quickly, cheaper and simpler compared to usual methods, besides that with a method standard for all the organs, this makes it possible to quantitatively compare the results for different organs.